Distribution of DArT, AFLP, and SSR markers in a genetic linkage map of a doubled-haploid hexaploid wheat population.

نویسندگان

  • Kassa Semagn
  • Asmund Bjørnstad
  • Helge Skinnes
  • Anne Guri Marøy
  • Yalew Tarkegne
  • Manilal William
چکیده

A genetic linkage mapping study was conducted in 93 doubled-haploid lines derived from a cross between Triticum aestivum L. em. Thell 'Arina' and a Norwegian spring wheat breeding line, NK93604, using diversity arrays technology (DArT), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. The objective of this study was to understand the distribution, redundancy, and segregation distortion of DArT markers in comparison with AFLP and SSR markers. The map contains a total of 624 markers with 189 DArTs, 165 AFLPs and 270 SSRs, and spans 2595.5 cM. All 3 marker types showed significant (p < 0.01) segregation distortion, but it was higher for AFLPs (24.2%) and SSRs (22.6%) than for DArTs (13.8%). The overall segregation distortion was 20.4%. DArTs showed the highest frequency of clustering (27.0%) at < 0.5 cM intervals between consecutive markers, which is 3 and 15 times higher than SSRs (8.9%) and AFLPs (1.8%), respectively. This high proportion of clustering of DArT markers may be indicative of gene-rich regions and (or) the result of inclusion of redundant clones in the genomic representations, which was supported by the presence of very high correlation coefficients (r > 0.98) and multicollinearity among the clustered markers. The present study is the first to compare the utility of DArT with AFLP and SSR markers, and the present map has been successfully used to identify novel QTLs for resistance to Fusarium head blight and powdery mildew and for anther extrusion, leaf segment incubation, and latency.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

اشباع نقشه پیوستگی ریزماهواره‌ای گندم نان درجمعیت حاصل از تلاقی Fukuho-Komugi × Oligo-Culm با استفاده از نشانگرهای AFLP

Genetic maps with high genome coverage are becoming increasingly useful in both basic and applied genetic researches. In the last decades, the advent of DNA markers has brought about a magnificent revolution in the production of genetic map, especially in wheat. In the present study, AFLP markers were used to saturate linkage map of 107 doubled haploid individuals produced through Fukuho _Komug...

متن کامل

اشباع نقشه پیوستگی ریزماهواره‌ای گندم نان درجمعیت حاصل از تلاقی Fukuho-Komugi × Oligo-Culm با استفاده از نشانگرهای AFLP

Genetic maps with high genome coverage are becoming increasingly useful in both basic and applied genetic researches. In the last decades, the advent of DNA markers has brought about a magnificent revolution in the production of genetic map, especially in wheat. In the present study, AFLP markers were used to saturate linkage map of 107 doubled haploid individuals produced through Fukuho _Komug...

متن کامل

The genetic study utility of a hexaploid wheat DH population with non-recombinant A- and B-genomes

To study the D-genome of the wild wheat relative Aegilops tauschii Cosson at the hexaploid level, we developed a synthetic doubled-haploid (DH) hexaploid wheat population, SynDH3. This population was derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from a cross between Triticum turgidum ssp. dicoccon PI377655 and A. tauschii ssp. strangulata AS66 × ssp. tau...

متن کامل

انتساب نشانگرهای AFLP به گروه‌های پیوستگی در لاین های هاپلویید مضاعف گندم

Molecular mapping and construction of linkage maps in plants genome play important role in marker assisted selection and breeding programs of target traits. Eleven combinations of PstI and MseI primers were used to assign AFLP markers to the wheat (Triticum aestivum L.) linkage groups in 107 doubled haploid (DH) lines. The results of AFLP analysis indicated that amplified fragments (66 polymorp...

متن کامل

Genetic Map of Triticale Integrating Microsatellite, DArT and SNP Markers

Triticale (×Triticosecale Wittm) is an economically important crop for fodder and biomass production. To facilitate the identification of markers for agronomically important traits and for genetic and genomic characteristics of this species, a new high-density genetic linkage map of triticale was constructed using doubled haploid (DH) population derived from a cross between cultivars 'Hewo' and...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Genome

دوره 49 5  شماره 

صفحات  -

تاریخ انتشار 2006